Yeast Two Hybrid Assay: a Fishing Tale

Solmaz Sobhanifar | Pages 81-88 | BioTeach Online Journal | Vol. 1 | Fall 2003

The yeast "two hybrid" or "interaction trap" assay, is a technique used to study protein-protein interactions, which are critical in virtually all cellular processes. The study of protein-protein interactions can be divided into three major parts: identification of binding proteins, the characterization of known interactions and the potential to manipulate such interactions...